Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Zfp410

Cell type

Cell type Class
Blood
Cell type
G1E-ER4+E2
Tissue
Blood
Lineage
cellLine
Description
Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Attributes by original data submitter

Sample

source_name
G1E-ER4
cell line
G1E-ER4 immortalized erythroid cell line
chip antibody
ZNF410 (proteintech, 14529-1-AP)
treatment
G1E-ER4 cells at 24 hours of differentiation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone/transcription factor-DNA complexes were isolated with antibody. Libraries were prepared according to standard Illumina protocols

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
49255196
Reads aligned (%)
89.0
Duplicates removed (%)
48.3
Number of peaks
700 (qval < 1E-05)

mm9

Number of total reads
49255196
Reads aligned (%)
88.9
Duplicates removed (%)
48.3
Number of peaks
721 (qval < 1E-05)

Base call quality data from DBCLS SRA