Antigen

Antigen Class

TFs and others

Antigen

Zfp410

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4

cell line

G1E-ER4 immortalized erythroid cell line

chip antibody

ZNF410 (proteintech, 14529-1-AP)

treatment

G1E-ER4 cells at 24 hours of differentiation

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone/transcription factor-DNA complexes were isolated with antibody. Libraries were prepared according to standard Illumina protocols

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

49255196

Reads aligned (%)

89.0

Duplicates removed (%)

48.3

Number of peaks

700 (qval < 1E-05)

mm9

Number of total reads

49255196

Reads aligned (%)

88.9

Duplicates removed (%)

48.3

Number of peaks

721 (qval < 1E-05)